This study explores the potential of unlocking ecotourism opportunities in Pench National Park through biodiversity conservation. By analysing the park’s rich biodiversity, including key species such as the Bengal tiger (Panthera tigris tigris), Indian leopard (Panthera pardus fusca), and numerous bird species, we examine the synergy between conservation efforts and ecotourism development. The study used a mixed-methods approach, combining field surveys, interviews with local stakeholders, and data analysis of wildlife population trends and tourist influx. Results indicate a 15% increase in wildlife sightings over the last three years, correlating with a 20% rise in ecotourism activities. Data from local communities highlight that 65% of households now benefit economically from tourism-related activities, showing that biodiversity conservation efforts directly support sustainable livelihoods. However, challenges such as habitat fragmentation and human-wildlife conflict were noted, necessitating more integrated conservation strategies. The findings demonstrate that Pench National Park has significant potential for ecotourism, but success hinges on continuous conservation efforts and stakeholder collaboration. Ecotourism, if developed responsibly, can provide a sustainable economic model while preserving the park’s ecological integrity, ultimately supporting both conservation and local development goals.