Background: Breast carcinoma (BC) issecond most prevalent form of cancer globally with infiltrating ductal carcinoma (IDC)being the most frequenthistologicalsubtype.Most hypotheses on cancer development emphasize the significance of the tumor microenvironment, namely the existence of cancer-associated fibroblasts, in the initiation and advancement of cancer. Myofibroblasts may be identified by employing an Immunohis to chemical marker called Alpha smooth muscle actin (α-SMA). Objectives: To determine the proportion of Alpha SMA in the stroma of Infiltrating ductal carcinoma of the breast and to correlate it with histopathological and hormonal expression. Materials and Methods: In this laboratory based observational study100 cases of IDC NOS were included. Following the immunohistochemistry analysis of Alpha SMA, the demonstration of SMA in stroma was examined in relation to various prognostic factors including age, histological grading, lymph node status, extra nodal extension, tumor staging, hormonal expression, and molecular subtype of the tumor. The study conducted descriptive statistics and evaluated the statistical significance of the difference between the two groups. The Chi-square test was used to evaluate categorical data. A p-value that is less than 0.05 was taken as statistically significant.