According to World Health Organization (WHO), the prevalence rate of superficial fungal infection and bacterial infection in worldwide has been found to be 20-25%. However, the increase in antibiotic resistant strains together with the lack of and high cost of new generation antibiotics increased woundrelated morbidity and mortality. So far, Herbal medicines are being used by about 80% of the world population mostly in the developing countries for primary health care. These medicines have stood the test of time for their safety, efficacy, cultural acceptability and lesser side effects. Traditionally used coconut shell liquid smoke a natural resources has been used as antibacterial and antifungal agent. The aim of this project is to analysis the  antibacterial, antifungal activity and gas chromatography massspectrometry of CS-LS Extract .In this study identification of the organism wea carried using convential method and species were confirmed by biochemical test,most of the orhanism were comes under Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and fungal organism Aspergillus niger, Aspergillus fumigatus, Candida albicans, Rhizopus species were done by conventional method. Antibacterial susceptibility was performed by Agar well diffusion method and Its components were identified by Phytochemical analysis using Gas Chromatography Mass Spectrometry for the identification of active compound. CS-LS showed zone of inhibition for bacterial and fungal organism except Rhizopus in the agar cup diffusion method. It was compared with standard drugs and antiseptic and can be used in the treatment of skin related infections. The phytochemical analysis relived the major organic component which can incorporated in disinfectant and antiseptic in future to overcome the hospital acquired infection.