Introduction: Diuretics can alleviate a number of pathological conditions, including elevated blood pressure, decreased fluid retention, edema, swollen ankles, and discomfort. Information regarding the characteristics and applications of medicinal plants is highly sought after due to the global trend toward the usage of natural plant treatments. Aim:For the aforementioned reasons, the present investigation’s objective is to assess the diuretic efficiency of the hydroalcoholic extract ofMticaria Chamomilla. Methodology: Five grams of the dried plant were roughly ground up and extracted using ethanol and water. After filtering, each aqueous extract was put to use. Histopathology and Diuretic activity are studied for the hydro-ethanolic extract of Matricaria chamomilla leaves. Result: When compared to zero days, the aforementioned particulars—likecreatinine, urea, and glucose—don’t alter during the seven-day treatment. Comparing the 15th day to the zero days, smaller biochemical alterations were seen. Urine volume increased in response to the Matricaria chamomilla hydroalcoholic extract as well. At 200 mg/kg and 400 mg/kg body weight, the increment was 18% (p < 0.01) and 41% (P < 0.001), accordingly, in contrast with thegroup under control. Conclusion: By using ion-selective channel blocking to measure the ions and electrolytes evacuated by the animals, this current investigation will be very helpful in determining the diuretic effect of the Matricaria chamomilla leaf extract.