Background: Ovaries are removed in variety of pathological conditions either alone or during hysterectomy or salpingectomy. So the present study was done to assess the ovarian functions in patients after hysterectomy with or without salpingectomy by using markers like sex hormones like estradiol, FSH, AMH. Aim: The comparison of ovarian function after hysterectomy with and without salpingectomy. Methodology: A Cohort study was done on 60 patients who admitted to gynecology ward, Vinayaka mission’s medical college, karaikal for a duration of 18 months. Females of age 30-50 years, whose USG does not show any ovarian pathologies and hysterectomy indicated for uterine pathologies with normal ovaries were included in the study. Females with ovarian dysfunction, incidental ovarian pathology findings intra-operatively H/E revealed malignancy were excluded from the study. Investigations namely S.AMH, FSH, Estradiol on day 3 of menstrual cycle Patients who undergoes hysterectomy with salpingectomy are divided in one group and without salpingectomy in other group. Investigations namely S.AMH FSH, Estradiol done at end of 1st and 3rd month postoperatively for both groups. Conclusion: Ovarian function is not affected by bilateral prophylactic salpingectomy in reproductive age women who underwent hysterectomy in our study. However further studies are needed to observe whether salpingectomy is effective and risk preventing choice of ovarian cancer in future