This study investigates the factors influencing the psychological well-being of employees in the Malaysian manufacturing industry, with a focus on stress, organizational culture, work-life balance, job burnout, and psychological well-being. Utilizing Structural Equation Modeling (SEM), data was collected from a sample of manufacturing employees to examine the relationships between these constructs. The results revealed significant negative associations between stress and psychological well-being, and significant positive associations between organizational culture, work-life balance, and psychological well-being. Additionally, job burnout was found to have a significant negative impact on psychological well-being. The findings underscore the importance of addressing stressors, cultivating a supportive organizational culture, promoting work-life balance, and mitigating job burnout to enhance employee well-being and organizational success in the manufacturing sector. These insights have implications for organizational leaders, policymakers, and human resource practitioners in fostering a conducive work environment that prioritizes employee mental health and well-being.