To determine a chemical's hazardous potential, antimicrobial activity research on several compound kinds is highly helpful. By adding different amounts of certain functionalized calix[4]pyrrole compounds to test organism culture, antibacterial activity has been evaluated. For known bacterial and fungal cultures, synthesised has been evaluated for a specific activity. As test organisms, Staphylococcus aureus, P. aeruginosa, S. pyogenes, and Escherichia coli bacterial subcultures as well as fungal subcultures of Candida albicans, Aspergillus nigricans, and Aspergillus clavatis have been utilised. At various concentrations, all materials were examined against these stains.