The present study was an attempt to assess religious orientation and psychological distress as well as to find out relationship of religious orientation with psychological distress among university students. University students were also compared on religious orientation and psychological distress with respect to their gender.  The data for the present study was obtained from university students [N=200 (Males 110 & Females 90)] belonging to various departments of Aligarh Muslim University. The data collected was analyzed by using appropriate statistical techniques like Pearson’s product moment correlation and t-test. The results showed significant negative correlation between intrinsic religious orientation and psychological distress. Moreover, a significant positive correlation was found between extrinsic religious orientation and psychological distress. Significant difference was also found among university students on extrinsic religious orientation with respect to their gender. However, no significant difference was found among them on intrinsic religious orientation and psychological distress with respect to their gender.