Introduction: To assess the knowledge, attitude and practice of rational use of antibiotics among undergraduate, intern and postgraduate dental students and make necessary interventions suitable to meet their needs and aspiration. Methods: A questionnaire was administered to the students of Rama dental college hospital & research centre to assess their knowledge, attitude and practice about the rational use of antibiotics. Data were analysed after entering into a Microsoft Excel sheet and using descriptive statistics.  Results: 135 dental students were included, with a response rate of 82%. Of the total participants, 120 (88.88%) know that Improper antibiotic use can cause antibiotic resistance. 56 (41.48%) participants agreed that antibiotics should be prescribed for all dental infections, while 79 (58.52%) denied this. Among all the participants, 48 (35.56%) participated in prescribing antibiotics because the patient expected it, while 87 (64.44%) said no. Conclusions: This study showed that most students had average knowledge of antibiotics. Their attitude could have been more satisfactory towards the rational use of antibiotics, and the same was true for the practice as well. Educational intervention is of utmost essential to improve their KAP regarding the sensible use of antibiotics.