Background: Dentistry has undergone a number of new developments that have forced it to advance based on evidence. The finest available research evidence is gathered to support good and successful dental practice, not on isolated pieces of evidence. This strategy creates a link between research and routine patient care. Aims & objectives: To assess and compare the knowledge, attitude, and perceived barriers toward evidence based practice (EBP) among faculty members associated with dental college and private practitioners in Kanpur city. Materials & Methods: A cross sectional-survey was conducted among 204 dentists (faculty members associated with these two private dental colleges and private practitioners) in kanpur city using a convenience sampling method. Data was collected through a validated, structured questionnaire which included demographic data, awareness, attitude, practices and barriers regarding EBD among the dentists. Results: In our study, based on the pre described grading for knowledge score, among the faculty members, 10(4.9%) showed poor knowledge, 17(8.3%) had fair whereas, only 67(32.8%) had good knowledge regarding EBP and for private practitioners, 66(32.4%) showed poor and 24(11.8%) fair knowledge regarding EBP and 20(9.8%) respectively. Among the participants, 55.4% of the participants showed positive attitude and 70(34.3%) practice EBD sometimes whereas, lack of access to full text articles was the most common barrier as reported by131(64.2%) and 19(9.3%) in the group 1 and 2 respectively. Conclusion The majority of the dentists in our survey had a good knowledge score but still understanding of the statistical terms used in EBP was limited whereas, dental professionals also showed a positive attitude towards EBP, but there are still some challenges to practice Evidence Based practice.