In mycotoxicology, TLC has developed into a very effective, quick, and in most cases, affordable separation procedure. This study involves screening of mycotoxins from some fungi like Aspergillus sp, Penicillium sp, Fusarium sp, Mucor sp, Nocardia sp, Trichoderma sp, Curvularia sp, Bipolaris sp, Rhizopus sp, Alternaria sp, throughThin Layer Chromatography (TLC) technique, an easy physicochemical laboratory process, to identify mycotoxins in bakery products. For the extraction of mycotoxins, various solvent systems were employed. The purpose of the further research on this work is to find out more about the specific mycotoxins present in the target fungal species.