Objective: The antibacterial activities were done by using of various solvents such as ethanol, petroleum ether, methanol, chloroform, n-butanol and ethyl acetate extracts of various marine sponges like Callyspongia reticutis Dendy (C. reticutis), Thalysias vulpine Lamark (T. vulpine), Echinodictyum gorgonoides Dendy (E. gorgonoides) and Callyspongia diffusa Ridley (C. diffusa), Gelliodes cellaria Rao (G. cellaria) against gram positive and gram negative bacterial pathogens like Pseudomonas aeruginosa PA01 and Proteus mirabilis ATCC 7002 respectively. Methods: The nutrient agar well diffusion method is used to find the antibacterialbial activities against various sponge extracts. The functional groups of the sponge extracts are specifically mitigate bacterial metabolic intermediates determined by FTIR. Results: The antibacterial activities of the sponge crude extracts were increased with different concentrations in the agar well plates to indicate the presence zone of clearance. The crude extracts were prepared from different solvents such as the n-butanol and chloroform extract was the most effective extracts. At this stage the gram negative bacteria Proteus mirabilis (P. mirabilis) and the gram positive bacteria Pseudomonas aeruginosa (P. aeruginosa) appear to be most sensitive strain while and Escherichia coli, Bacillus cereus, Klebsiella pneumonia. The Staphylococcus aureus indicate resistance to the various tested concentrations and have response to no zone of inhibition was observed. The inhibition of microbial growth at concentration as low as ~50 -150 mg/mL indicated the potent antibacterial activity of above mentioned porifera sponge extracts. Conclusions: These research works results were find critically industrially important compounds from selected sponge extracts and the functional groups of plant compounds is responsible for great antibacterial activity.