The present investigation, conducted between 2022 and 2025 at IIS (Deemed to be University), aimed to evaluate the efficacy of homeopathic remedies Natrum muriaticum 6CH and 12CH in alleviating saline stress induced by 100 mM NaCl in Pennisetum glaucum (L.) R. Br. under in vitro conditions. The remedies were incorporated into Murashige and Skoog medium supplemented with appropriate hormones BAP and 2,4-D for callus induction, and IAA with kinetin for regeneration. Seed germination, callus formation, and regeneration were initiated in 11, 10, and 3 days respectively under treatment with the remedies. Morphological parameters such as root-to-shoot ratio and callus biomass, along with biochemical markers including chlorophyll a, chlorophyll b, total chlorophyll, total phenolic content, and DPPH radical scavenging activity, showed significant improvement. Gene expression analysis through quantitative RT PCR revealed modulation of stress-responsive genes (NAC21 and APX) with ACTIN as the reference, confirming upregulation under treatment. Statistical analysis validated the reliability of these findings. Overall, both potencies of Natrum muriaticum demonstrated efficiency in ameliorating NaCl-induced salt stress, thereby enhancing seed germination, callus formation, and regeneration in pearl millet.