Flax is an important source of oil rich in omega-3 fatty acid which is proven to have health benefits and utilized as an industrial raw material. An application was observance to estimate the volume of inherited deviation among 33 flaxseed genotype including 3 checks using Mahalanobis D² statistics in Randomized block design (RBD) at Agriculture Research Institute Kanpur, Up during Rabi 2022-2023. Examination was record for 11 ethos and based on D² statistics, the genotype were classed into 8 apart team using Tocher’s method. The outgrowth visible that team I had most number of genotypes followed by team II and team VI while other five team were mono-genotypic. The higher intra-team remoteness was recorded for team VI, followed by team II and team I while the lowest intra-teamremoteness were observed for remaining 5 team. Whereas the most inter-team remoteness was recorded between team IV and VIII, indicating a higher amount of inheritedmulteity available in genotypes of this team and can be harness as parents for inter-breeding schedule.