Background: Flexibility is the ability to move a single joint or a group of joints efficiently over a painless, unrestricted Range of Motion (ROM). Reduced flexibility may result in a diminished range of motion, which in turn alters the biomechanics and, as a result, the joints. Maintaining a prolonged forward bend sitting position causes strain on the hamstrings, which leads to decreased flexibility. Objectives: To examine the combined impact of static stretching, the suboccipital muscle inhibition technique (SMI), and the myofascial release technique (MFR) on hamstring flexibility, as measured by an active knee extension test, both before and after the intervention. Methodology: The 45 participants were divided into three groups according to the selection criteria. Myofascial release method was given to the 15 people in group A, while Sub occipital inhibition technique was administered to the 15 people in group B. And Group C was given Static stretching and had 15 participants. For four weeks, all of the groups underwent the interventions five times a week. Results: Active knee extension test was used to measure hamstring flexibility during the pre- and post-tests. For AKE(R), the pre-mean values of groups A, B, and C were 120.07, 120.65, and 119.43, respectively. The pre-mean value of group A, B, and C for AKE (L), where 119.27, 119.2, and 119.8, and AKE(R), where 140.47, 147.53, and 132.93, are the Post mean values for groups A, B, and C. The Post mean group A, B, and C for AKE (L) have values of 139.6, 146.4, and 131.73, respectively. Conclusion: In summary, the research found that the Sub occipital muscle inhibition method was more successful in increasing hamstring flexibility in IT workers who had hamstring tightness.