Oyster mushrooms, belonging to the Pleurotus genus within the Class Basidiomycetes and Family Agaricaceae, are known for their ability to thrive on decomposing organic matter. The fruiting bodies of these mushrooms typically exhibit a shell- or spatula-shaped appearance and can vary in color, ranging from white, cream, and grey to yellow, pink, or light brown, depending on the species.A study was conducted to evaluate the effects of various manure-based composts on the growth, yield, and nutritional composition of Pleurotus florida. The mushrooms were cultivated on different substrates, including rice straw mixed with cow dung compost (1:1) (T2), sheep dung compost (1:1) (T3), poultry manure compost (1:1) (T1), cow urine compost (1:1) (T5), silkworm dung compost (1:1) (T4), and panchakavya compost (1:1) (T6). Compost preparation involved a mix of rice straw (60%), rice bran (5.5%), chicken manure (31%), urea (0.5%), and gypsum (3%).The results revealed significant variations in growth, yield, biological efficiency, and the nutritional profile of the mushrooms across the different substrate formulations. Notably, substrates without compost supplementation demonstrated superior performance in terms of yield and biological efficiency. Among the compost treatments, the temperature of the substrates rose from an initially low level to peak values on the fourth day, reaching 69°C (T1), 59°C (T2), 54°C (T3), 61°C (T4), 65°C (T5), and 63°C (T6). The highest yield of Pleurotus florida was recorded at 443 grams.