Hericiumerinaceuscan strengthen the spleen, nourish the stomach, calm the mind, and combat cancer. The studies were carried out in vitro to investigate the possibilities of various medium, temperature, pH and Light intensity. Present study carried out on two strains of H. erinaceous (DMRX-779 and DMRX-780) for evaluation of their cultural characteristics on different media, temperature, pH and light intensity. Among the selected media PDA and WEA showed better mycelia growth. For analysis of temperature (25ºC) were recorded best followed by 20ºC temperature for both the strains and variable pH (5, 6, 8 and 7) was recorded. Light serves as a significant growth factor that has been assessed for the growth of selected strains for the first time. Yellow light promoted fruiting bodies initiation while blue light favors rapid colonization in petriplates. Both strains could be further improved for commercial and economic purposes based on these considerations.