The study was aim to investigate various agro-wastes (Sawdust, wheat straw, paddy straw, sorghum straw, finger millet straw and pearl millet straw) in mixture found locally for cultivation of Lentinula edodes-327.With respect to this 5 substrates were tested on the basis of growth parameters and yield performance. Results based on the current study revealed that T1 had the shortest spawn run and bump formation time (54.75 days 32.50 days), while T1 display shortest browning period time (8.50 days). The minimum total incubation time was 95.75 and 106.00 days by T1 and T2, Stipe diameter was maximum in T3 (2.46 cm) and minimum in T5 (1.41cm). T1 had the longest stipe and cap diameter of 4.32 cm and 8.24 cm respectively whereas T1 had the thickest cap (3.54 cm). The highest yielding substrate in 1^st flush was T3 (287.7 gm), 2^nd flush (175.23 gm) and 3^rd flush (107.70 gm).The highest biological efficiency rate is 28.54% by T3 followed by T1 (22.46%).