Generally we can find gender differences in many features of human life. Especially in this study, the main variables such as Self-Acceptance and Prosocial Behaviour may displaymuch differences in the aspect of gender.In our daily life we may experience that, male children have somewhat better Self-Acceptance and shows more Prosocial Behaviour than female children. This study aimed to understand, whether the college students have the same attribute at this period of transition from adolescence to adulthood in the modern era? With this aim, the investigator, used self-made tools such as “Self-Acceptance” and “Prosocial Behaviour” after standardizing the same. By adopting descriptive survey method, along with a personal data sheet, these two tools have been distributed to 321 students who are studying in various streams. Statistical analysis has been made to attain the goal of this investigation. The result shows that, the boys are better than the girl students in both Self-Acceptance and Prosocial Behaviour. And there is also a significant association exist in both Self-acceptance and Prosocial Behaviour with their Mode of Survival and Income Generated per month. Students who are living with single parents and also who have Income between Rs.20,000/- and Rs.50,000/- per month are better in Self-Acceptance and Prosocial Behaviour. Generally girls never accept themselves as they are. They usually compare with others and feeleither inferior or superior. When they areready to accept themselves as they are,they will involve themselves muchin many social activities.Hence, it is suggested that, some kind of external motivation is required for them.By appreciating their achievements, they can improve their Self-Acceptance and they will become a socially responsive person.