The aim of this paper is to determine the relationship between working environment and job satisfaction in banking sector in the city of Kolkata, India. Simple random sampling is used for collection of data. The standard structure questionnaire is administered for collection of data from 230 employees working in banking sector in the city of Kolkata.The standard structured questionnaire named “Working Environment (WE) Scale”designed and developed bySpector (1997), and Job Satisfaction (JS) Scale designed and developed by Weiss et al. (1967) was administered. The hypotheses formulated in the study were analysed by using appropriate statistical test like mean, SD, t-test, ANOVA and regression analysis. The results reveals that the working environment and job satisfaction is positively correlated and has a positive relationship between working environment and job satisfaction of the employees in banking sector. The study concludes that the organisation needs to understand the importance of the healthy working environment for achieving the highest level of job satisfaction of the employees.High level of job satisfaction leads to motivate their employees and encourage to work hard for attaining the goals and objectives of the organisation.